TABLE 1.
Strains used in this study
| Strain No. | Strain description | Relevant ActA sequenceb | VASPc |
|---|---|---|---|
| 10403Sa | Wild type | 263:DFPPPPTDEEL-LR1-EFPPPPTDEEL-LR2-EFPPPPTEDEL-LR3-DFPPIPTEEEL:390 | ++++ |
| DP-L3903 | Reference | 263:DFPPPPTDEEL-LR1-EFPPPPTDEEL-LR2-EFPPPPTEDEL-LR3-DFPPIPTEEEL:390 | ND |
| DP-L2300 | ΔActA3 | 263:DFPPPPTDEEL-LR1-EFPPIPTEEEL:390 | ++ |
| DP-L2990 | ΔActA3-GG | 263:DFGGGGTDEEL-LR1-EFGGGGTEEEL:390 | − |
| DP-L2374 | ΔActA6 | 263:390 | − |
a
10403S, DP-L2300, DP-L2990, and DP-L2374 are described in reference 22.
b
Underlined residues indicate where prolines have been substituted with glycines. Sequences shown begin at position 263 and end at position 390. LR, long repeated sequence.
c
Quantification of VASP recruitment to intracellular bacteria using indirect immunofluorescence as reported in reference 22. ++++, greatest relative fluorescence; −, no fluorescence detected. ND, not determined.