Figure 2.

Maltodextrin can be effectively used as both an energy source and lyoprotectant for low-cost CFE. (A) Cost per mL CFE reaction was calculated for each formulation: PEP with no lyoprotectant, PEP with maltodextrin supplemented as a lyoprotectant (PEP MD), maltodextrin as an both energy source and a lyoprotectant (MD), and maltodextrin without CoA, tRNA, and replacing NTPs with NMPs (MD min). Costs are based only on raw materials included in the reaction purchased at laboratory scale using calculations in Supporting Tables S1–S3. (B) Cost per milligram sfGFP in CFE reactions using BL21 Star (DE3) extract in all four formulations. (C) Cost per milligram sfGFP in CFE reactions using CLM24 ΔlpxM extract in all four formulations. Error bars represent the standard deviation of three CFE reactions (n = 3).