Table 1.

Insulin and pramlintide peak parameters eluted from different columns.

Column type	Rs Insulin-pramlintide	Tf insulin	Tf pramlintide	As insulin	As Pramlintide
Novapak 150 mm	Pramlintide ND	3.5	ND	4	ND
Novapak 250 mm	Pramlintide ND	1.57	ND	2	ND
μ-Bondapack 250 mm
In mobile phase	2.32	1.2	1.11	1.2	1.18
In loading medium	2.81	1	1.07	1.03	1.2
In release medium	2.81	1.1	1	1.23	1.07
ICH acceptable range (17)	> 1.5	1-1.5		1-1.25

Tf, Tailing factor; Rs, resolution factor; As, asymmetry factor; ND, not detected; ICH, International Conference on Harmonization.