Table 5.

Intra and inter-day variations of the HPLC method for determination of insulin and pramlintide in the release medium

Insulin									Pramlintide
Concentration (μg/mL)	Intra-day precision and accuracy				Inter-day precision and accuracy					Intra-day precision and accuracy				Inter-day precision and accuracy
	Mean	SD	CV (%)	Error (%)	Mean	SD	CV (%)	Error (%)	Concentration (μg/mL)	Mean	SD	CV (%)	Error (%)	Mean	SD	CV (%)	Error (%)
30	26.89	0.43	1.6	10.35	27.66	0.27	1.00	7.80	1.5	1.64	0.01	0.92	9.33	1.62	0.008	0.51	8.00
60	57.82	0.01	0.02	3.63	57.62	0.05	0.10	3.96	2	2.43	0.07	3.15	21.5	2.36	0.07	2.24	18.00
105	106.80	0.07	0.07	1.71	105.10	0.54	0.52	0.09	3.5	3.27	0.09	3.02	6.57	3.20	0.099	3.08	8.57
150	147.06	3.6	2.5	1.96	153.57	3.36	2.19	2.38	5	4.65	0.08	1.74	7.00	4.59	0.08	1.78	8.20
195	204.01	6.21	3.04	4.60	204.99	5.46	2.56	5.12	6.5	6.42	0.11	1.71	1.23	6.39	0.15	2.39	1.69
240	242.80	4.9	2.03	1.16	243.56	6.09	2.32	1.48	8	8.23	0.11	1.43	2.87	8.27	0.15	1.88	3.37
300	297.76	3.03	1.01	0.74	299.18	3.30	1.10	0.27	10	9 85	0.07	0.73	1.50	9.92	0.07	0.72	0.80
360	356.02	0.24	0.06	1.11	356.15	5.69	1.60	1.07	12	12.18	0.004	0.04	1.50	12.11	0.10	0.90	0.91

SD, standard deviation; CV, coefficient of variation.