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. 2022 Apr 15;72(2):345–359. doi: 10.1136/gutjnl-2021-325564

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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LOXL2 mRNA expression correlates with poor overall survival and EMT in patients with PDAC. (A) Differential expression of LOXL2 in adjacent (Adj.) normal tissue vs PDAC tumours and metastasis (met) in GSE62165, META data set, and GSE71729. Unpaired two-sided Student’s t-test. (B) LOXL2 relative mRNA levels ±SD (n=2 technical replicates) in a panel of surgically resected human PDAC tumours (n=25) and four normal pancreas (nPanc) controls (left). Pooled mean ±SEM analysis including four primary KPC tumours (right). (ns, not significant; ****p<0.0001; two-sided t-test with Mann-Whitney U test). (C) Overall survival of patients with PDAC from the Bailey (n=96) data set, stratified according to the median value of LOXL2 expression. HR=Hazard ratio, Cox proportional hazard regression model. A Log-rank test was performed for survival analysis. (D) Differential expression of LOXL2 in PDAC tumours, subtyped as progenitor, squamous, immunogenic or ADEX from the Bailey et al data set. (**P<0.01, ****p<0.0001, one-way analysis of variance with Dunnett post-test). (E) EMT pathway enrichment plots from transcriptomics analysis (GSE62165, META and GSE1729 data sets) of LOXL2 high vs LOXL2 low patients. FDR <0.25. (F) Pearson correlation matrix of mesenchymal-related and epithelial-related genes in 179 patients with human PDAC (TCGA) sorted for LOXL2 mRNA levels and nearest neighbour. The matrix was subjected to supervised hierarchical clustering (Euclidean distance measurement, average linkage clustering). EMT, epithelial to mesenchymal transition; FDR, false discovery rate; LOXL2, lysyl oxidase-like protein 2; mRNA, messenger RNA; PDAC, pancreatic ductal adenocarcinoma; TCGA, The Cancer Genome Atlas.