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. 2022 Mar 3;72(2):275–294. doi: 10.1136/gutjnl-2021-325520

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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Analysis of cell shedding and overcrowding in small intestine sections from human patients with IBD. (A–C) F-actin fibre staining using AlexaFluor488-phalloidin (green) (n=13, total; n=5, Control; n=8, CD). (A) Representative pictures (top); yellow arrows indicate epithelial gaps; orange arrow indicates microerosions. (B, C) Quantification. (B) Gap length (% of epithelial length), and mean gap length (µm/gap). (C) Funnel-like structures (red triangles) (% of total cells) and calculated length/diameter ratio (AU). (D, E) 2D/monolayer organoids generated from human intestinal crypts (three patients), and treated with NSC-23766 (100 µM) or a cytokine cocktail (IL-1β 10 ng/mL, IL-6 10 ng/mL and TNF-α; 20 ng/mL). (D) Phalloidin staining. (E) E-cadherin staining. Paired t-test. *P≤0.050. IBD, inflammatory bowel disease.