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IL-1β (A) and IL-6 (B) production by J774 cells treated with viable or heat-killed B. henselae at a 10:1 bacteria/macrophage ratio and cultured for the indicated times. Culture medium was used as a negative control, and LPS (1 μg/ml) was used as a positive control. The data represent the means ± standard errors (error bars) from three independent experiments. Asterisks indicate significant increase of cytokine production compared to unstimulated cells (P < 0.05).
