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. 2023 Jan 23;15:12. doi: 10.1186/s13148-023-01430-y

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression levels of H3K27me3 and EZH2 in BALF of patients with wheezing after pulmonary inflammation. A Histone H3K27me3 in the BALF was detected by immunofluorescence staining. Red (H3K27me3), blue (DAPI). The scale bars indicate 20 μm. B Average optical density of H3K27me3 immunofluorescence in the three groups (n = 3). C The expression level of histone H3K27me3 in the BALF was detected by Western blotting (n = 10). D The expression level of EZH2 in the sediment of BALF was detected by immunofluorescence staining. Red (EZH2), blue (DAPI). The scale bars indicate 20 μm. E Average optical density of EZH2 immunofluorescence in the three groups (n = 3). F The mRNA expression of EZH2 in the BALF was detected by RT-qPCR (n = 10). G The expression level of EZH2 in the BALF was detected by Western blotting (n = 10). *P < 0.05 versus the Control group and Non-wheeze group