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(A) Chemical biology strategy to visualize and identify DPAs. (B) Synthesis of DAyne. (a) Methyl trifluoroacetate, triethylamine, CH3OH; 93%. (b) pTSA, 2,2-dimethoxypropane, toluene; 79%. (c) LiOH, CH3OH; 98%. (d) Nosyl chloride, triethylamine, CH2Cl2; 97%. (e) Propargyl bromide, K2CO3, acetone; 96%. (f) Thiophenol, KOH, CH3CN; 50%. (g) Trifluoroacetic acid, CHCl3 then HCl, tetrahydrofuran; 96%.
