Figure 4.

DA and DA^yne reduce SH-SY5Y cell viability. Cell viability was determined through an alamarBlue assay. Data are represented as mean ± SEM from four replicates and normalized to DMSO control. All treatments of DA and DA^yne at analogous times and concentrations were analyzed via a one-way ANVOA and an unpaired t-test (***p < 0.001). Percent viability of cells was determined following treatment with (A) DA or (B) DA^yne alone. (C) Cells were treated with 100 μM DA or DA^yne in the presence or absence of 2.5 mM NAC, which showed that cell viability was rescued under these conditions.