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. 2023 Jan 10;13:1074762. doi: 10.3389/fimmu.2022.1074762

Figure 2.

Figure 2

Flow cytometric analysis of TIDC subsets showing that perioperative corticosteroid treatment inhibits intratumoral DC recruitment and activation. (A) Frequency of DC subsets in healthy tissues (heathy controls: HCs, n=3) and tumor tissues (Gliomas, n=10) obtained from glioma patients. (B) Frequency of TIDC subsets in glioma patients either untreated (Untreated, n=5) or treated with dexamethasone (Dex-treated, n=5). Data expressed as per-thousand (‰) of CD45br cells. Each symbol represents a single sample. In each series, the mean is shown. *p<0.05, **p<0.01, ***p<0.001, calculated using the t-test. (C) UMAP plots showing the clustering of TIDC subsets in untreated and dex-treated glioma patients. Each plot shows a single DC subset as identified with manual gating strategy. Viable tumor-infiltrating CD45br/lin−/HLA-DR+ cells of down-sampled, concatenated files obtained from all glioma patients are shown in gray. pDCs are highlighted in dark turquoise, cDC1s in brown, cDC2s in orange, slanDCs in red, and moDCs in green. (D) Expression of HLA-DR, activatory molecules (CD40, CD80, CD86), and inhibitory molecules (PD-L1, ILT2) on each DC subset, expressed as MFI measured on concatenated files, and compared between untreated and dex-treated glioma patients.