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. Author manuscript; available in PMC: 2023 Jan 24.
Published in final edited form as: Nat Struct Mol Biol. 2022 Nov 7;29(11):1101–1112. doi: 10.1038/s41594-022-00853-0

Extended Data Fig. 9: XRN2 and SAM68 promotes cell cycle progression through APA modulation (Related to Fig. 7).

Extended Data Fig. 9:

From: The transcriptional terminator XRN2 and the RNA-binding protein Sam68 link alternative polvadenvlation to cell cycle progression in prostate cancer

a,b, Cell cycle (a) and sub-Gl (b) distribution assessed by PI staining in asynchronous LNCaP cells stably depleted for Sam68 and XRN2. c, Cell cycle distribution assessed by PI staining in asynchronous LNCaP cells stably depleted for MCM10 and ORC2. a-c, Data represent mean ± SD of three biological replicates. Statistical significance was calculated by unpaired Student’s t-test, two-sided. In a, the p-values are: Gl: sh-Sam68/sh-scr P = 2.2 × 10−3, sh-XRN2/sh-scr P = 8.6 × 10−3; S: sh-Sam68/sh-scr P = 2.0 × 10−4, sh-XRN2/sh-scr P = 1.2 × 10−3; G2-M: sh-Sam68/sh-scr P = 0.037, sh-XRN2/sh-scr P = 0.036; in b, sh-Sam68,/sh-scr P = 0.2264, sh-XRN2/sh-scr P = 0.6388; in C, Gl: si-MCM10/si-scr P = 2.6 × 10−6, si-ORC2/si-scr P = 3.6 × 10−3; S: si-MCMIO/si-scr P = 3.0 × 10−4, si-ORC2/si-scr P = 0.1679; G2-M: si-MCMlO/si-scr P = 2 × 10−4, si-ORC2/si-scr P = 0.0917). In the representation of panels, statistical value is reported as *P < 0.05; **P < 0.01; ***P < 0.001.