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Western blot for RelA in nuclear extracts obtained from lung tissues harvested 90 min following treatment with 20 μg of i.p. LPS per g. Results for p47phox−/− (knockout [KO]) mice are shown in lanes 1 to 5, and those for WT mice are shown in lanes 6 to 9. p47phox−/− animals had reduced levels of nuclear RelA compared to WT mice.
