FIG 1.

cAMP production by M. tuberculosis and Rhodococcus jostii RHA1 expressing Rv1625c treated with GSK286 (A) LC-MS total ion chromatogram from M. tuberculosis lysates following GSK286 treatment. 8-Br-cAMP (8-bromoadenosine 3′,5′-cyclic monophosphate) was supplied to M. tuberculosis as a control and run as a standard for LC-MS/MS analysis. (B) WT and an rv1625c transposon mutant of M. tuberculosis CDC1551 were grown in 7H9 medium supplemented with 0.2% glycerol, concentrated, and then incubated in 7H9 medium supplemented with 0.5 mM cholesterol with or without 5 μM GSK286. Cells were harvested after 24 h, and cell lysates were analyzed by HPLC. (C) R. jostii RHA1 carrying pTip1625 or empty pTip plasmid was grown in M9 medium supplemented with 0.5 mM cholesterol with or without 5 μM GSK286. Cell lysates were analyzed by HPLC. Data represent the means of three biological replicates.