FIG 3.

Inoculation with W-Hz confers full protection against a sporozoite infection. (A and B) BALB/c WT mice were inoculated i.v. with 750 nmol of W-Hz before a 50 Pb-ANKA-Luc sporozoite infection 1 week later. Parasitemias were followed over the course of 13 days postmerogony. (A) 5/5 mice inoculated with W-Hz were fully protected. Each data point corresponds to the group’s mean. Error bars correspond to the SEM. n = 10. (B) 4/5 mice were fully protected. Each data point corresponds to an individual mouse. There was a 2-day delay in patency between the infected mouse inoculated with W-Hz and the naive controls. n = 10. (C) Mice inoculated i.v. with 750 nmol of W-Hz 1 before a 2,000-sporozoite infection showed a 2-day delay in patency compared to the naive group. Each data point corresponds to the group’s mean. Error bars correspond to the SEM. n = 8. (D) Groups of BALB/c WT mice were inoculated i.v. with 750 nmol of W-Hz before a 2,000-Pb-ANKA-Luc sporozoite infection 1 week, 1 day, or 1 h later. Parasite liver load was measured 42 h postinfection in photons per second (total flux). Error bars correspond to the SEM. The nonparametric, unpaired Mann-Whitney test showed that there was a statistically significant difference between the naive control group and the groups inoculated with W-Hz 1 week, 1 day, or 1 h before sporozoite infection. ***, P ≤ 0.001; n = 20. (E) Parasitemias were followed over the course of 13 days postmerogony in the naive group, and the groups infected 1 day or 1 h after W-Hz inoculation. 5/5 mice in the 1-day W-Hz group were fully protected, and 5/5 mice in the 1-h W-Hz group were fully protected. Each data point corresponds to the group’s mean. Error bars correspond to the SEM. n = 15.