FIG 5.

Effect of ribavirin mutagenesis on the complexity of mutant spectra of SARS-CoV-2 populations. The diversity indices are those previously defined (50). Abbreviations (written at the top empty, elongated boxes) are as follows: Hpl, number of haplotypes; dHpl (Hpl/N), number of haplotypes normalized to the number of reads; nM, number of mutations; Hs, Shannon entropy; HsN, Shannon entropy normalized to the number of reads; HsH, Shannon entropy normalized to the number of haplotypes; Hsi, Simpson index; Hgs, Gini-Simpson index; ¹D, Hill number for q = 1; ²D, Hill number for q = 2; Mfmax, maximum mutation frequency; Mfmin, minimum mutation frequency; and π, nucleotide diversity. Bars represent the average ratios of the four nsp12 (polymerase) amplicons or the two spike amplicons (indicated in the filled boxes) (value obtained in the presence of Rib divided by the corresponding value in the absence of drug). The statistical significance of the increase of the values (Rib versus no drug) was a P value of <0.05 for all comparisons, except for the difference in Mfmax and π for Rib 100 μM and in Hsi that did not reach statistical significance (Wilcoxon test).