Fig. 2.

PolyADP-ribosylation of human ADH1 with [³²P]NAD⁺ in vitro Human ADH1 protein was incubated with [³²P]NAD⁺ under polyADP-ribosylation reaction conditions; with native or heat-inactivated PARP1 without or with 3AB. These samples were subjected to SDS-12.5% polyacrylamide gel electrophoresis followed by autoradiography (left panel) and silver staining (right panel). Histone H1 is a positive control for polyADP-ribosylation. Data are representative of three experiments.