FIG 1.

Distribution of accessible and total E-cadherin in undifferentiated and differentiated Caco-2 cells. Three-dimensional projections of a representative confocal micrograph of healthy undifferentiated (A) and differentiated (B) Caco-2 cells immunostained for accessible E-cadherin. Accessible E-cadherin (shown as acc Ecad) is in green, F-actin in red, and nuclei in blue. (C) Repartition of cells that were positive (Ecad+) or negative (Ecad−) stained for accessible E-cadherin. (D and E) Three-dimensional projection of a representative confocal micrograph of healthy undifferentiated (D) and differentiated (E) Caco-2 cells immunostained for total E-cadherin. (F) Repartition of cells that were positive (Ecad+) or negative (Ecad−) stained for total E-cadherin. Fluorophores colors were digitally reassigned for a better representation. Micrographs are representative of 6 fields of 2 independent experiments. Scale bar, 20 μm. Error bars indicate mean ± SEM. Statistical analysis was performed by two-tailed unpaired Student’s t test. ns, P > 0.05; ****, P ≤ 0.0001.