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Detection of capsular hyaluronic acid (HA) production. The quantification of the hyaluronic acid content in the wild-type strain, mutant strain, and complemented strain at logarithmic phase (OD600 of approximately 0.6) with three replicates per group. Data are expressed as the mean ± standard deviation from three independent experiments. **, P < 0.01; ***, P < 0.001. The experiments were performed at least three times.
