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. 2023 Jan 24;6:98. doi: 10.1038/s42003-023-04478-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The clusters were labeled according to their markers and only neuronal and glial cell markers were verified by in situ hybridization in the pig proximal colon (p-pC) a and pig distal colon (p-dC) b. Uniform Manifold Approximation and Projection (UMAP) visualized 10 distinct cell clusters and the cell-cell interaction (CCI) strength between neurons and glial cells was highlighted only. Color represents a CCI strength distribution from maximum (green) to minimum (white). The distinct cell clusters were derived from 2091 cells of one p-pC individual and from 3918 cells of one p-dC individual. Joint visualization based on four individuals of p-pC and p-dC could be found in Supplementary Figure 3. Violin plots depict the distribution of gene expression levels for 3 neuronal subpopulations c and 2 glial subpopulations d. Interactions (arrows) from ligand- to receptor-expressing neuronal and glial subsets e. Blue, neuronal subset; Black, glial subset. Growth associated protein 43 (GAP43), a marker for annotating pan-neurons; Claudin 11 (CLDN11) for pan-glial cells; ATP citrate lyase (ACLY) for cholinergic neurons; Glutaminase (GLS) for glutamatergic neurons; Rho GTPase activating protein 18 (ARHGAP18) for nitrergic neurons; Solute carrier family 41 member 1 (SLC41A1) and SPC24 component of NDC80 kinetochore complex (SPC24) for two subtypes of glia.