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. 2023 Jan 24;6:98. doi: 10.1038/s42003-023-04478-x

Fig. 5. Validation of discriminatory marker genes for major classes of enteric neurons and two subtypes of enteric glial cells (EGCs) in the myenteric ganglia of porcine proximal colon using RNAscope in situ hybridization.

Fig. 5

Confocal images were generated from 5–10 optical sections (Z-stack) cross the myenteric ganglia with frame 436 × 436 μm and 1 μm apart (20× objective). The co-expression of mRNA for GAP43 (annotating enteric neurons) with ELAVL4 encoding Hu C/D a, b, ACLY (annotating cholinergic neurons) with ChAT d, e, ARHGAP18 (annotating nitrergic neurons) with NOS1 g, h, GLS (annotating glutamatergic neurons) with SLC17A6 encoding VGLU2 j, k, CLDN11 (annotating EGCs) with GFAP m, n, and SPC24, SLC41A1 (annotating two subtypes of EGCs) with GFAP p, q, r were indicated in the merged images c, f, i, l, o and s, respectively. Scale bars: 20 µm.