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. 2023 Jan 24;18(1):e0280936. doi: 10.1371/journal.pone.0280936

Fig 1. Illustration of the quantification of newly formed lymph vessels in a SLN metastasis of an invasive breast carcinoma NST with LVD and CC.

Fig 1

The number of D2-40 stained newly formed lymph vessels was counted in five HPF in five different hot spots of D2-40 staining within the metastasis in a SLN, indicated as lymphatic vessels per high power field (LV/HPF) (A1-E1), and the mean was calculated (in Fig 1 19.6 LV/HPF). Correspondingly, the number of points in the Chalkley graticule superposed with D2-40 positive lymph vessels was counted, indicated as CC (A2-E2), and the mean was calculated (in Fig 1 4.4 CC). A1-E1, 400x magnification; A2-E2, 200x magnification.