Adherence of B. pertussis to cell lines. Human pulmonary epithelial cells (A549), murine alveolar macrophages (MH-S), primary cultures of dendritic cells (dendritic), and human tracheal cells (HEp-2) were incubated with the indicated 35S-labeled B. pertussis strains for 1 h 30 min at a multiplicity of infection of 20. After washing, adherence was estimated by scintillation counting. The results are expressed as percentages of counts per minute relative to the counts per minute present in the inoculum. The data represent averages and standard deviations for quadruplicate experiments. ∗, P < 0.05.