Figure 3.

Expression of GluN2B variants interferes with dendrite morphogenesis. (A) Primary neuronal cultures were prepared from wild-type rat cortex, at postnatal day 0–1 (P0–1), then transfected at 2 days in vitro (DIV) with either wild-type GluN2B or GluN2B bearing a truncation at amino acid 724 (724t), within the LBD (Sceniak et al., 2019; Bahry et al., 2021). In parallel, GluN2B with a large depletion in the LBD was also examined, producing similar results. (B) In contrast to neurons expressing only wild-type GluN2B, neurons expressing mutant GluN2B had impaired dendrite development, with some neurons appearing under-developed and others appearing dysmorphic (displaying unusual dendritic structures). Overall, GluN2B variants produced fewer branches and reduced branch length. Illustrations were partially created with BioRender.com. Images in (B) are from Sceniak et al. (2019).