| Strain, strain background |
C57BL/6J: Ikbkbtm2Cgn (mouse) |
This paper |
Ikbkbtm2Cgn |
Originally generated by Manolis Pasparakis (Pasparakis et al., 2002) |
| Strain, strain background |
C57BL/6J: Ikbkbtm2Cgn (mouse) |
Jackson Laboratories |
RRID:IMSR_JAX:008242
|
|
| Strain, strain background |
C57BL/6J: Tg(ACTA1-cre/Esr1*)2Kesr/J (mouse) |
Jackson Laboratories |
RRID:IMSR_JAX:025750
|
|
| Antibody |
Anti-myosin heavy chain (slow, alpha-, and beta-) (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
BA-F8 |
IF (1:30) |
| Antibody |
Anti-myosin heavy chain type IIA (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
SC-71 |
IF (1:30) |
| Antibody |
Anti-myosin heavy chain type IIB (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
BF-F3 |
IF (1:30) |
| Antibody |
anti-laminin (rabbit polyclonal) |
Abcam |
11575 |
IF (1:400) |
| Antibody |
Anti- SQSTM1/p62 (rabbit polyclonal) |
Cell Signaling Technology |
5114 |
IF (1:100), WB (1:1000) |
| Antibody |
Anti-LC3A/B (rabbit monoclonal) |
Cell Signaling Technology |
12741 |
IF (1:100), WB (1:1000) |
| Antibody |
Anti-PDGFRα (goat polyclonal) |
R&D Systems |
AF1062 |
IF (1:400) |
| Antibody |
Anti-Pax7 (mouse monoclonal) |
Developmental Studies Hybridoma Bank |
Pax7 |
IF (1:100) |
| Antibody |
Anti-IKKβ (rabbit monoclonal) |
Cell Signaling Technology |
8943 |
WB (1:1000) |
| Antibody |
Anti-mTOR (rabbit monoclonal) |
Cell Signaling Technology |
2983 |
WB (1:1000) |
| Antibody |
Anti-phospho-mTOR (Ser2448) (rabbit monoclonal) |
Cell Signaling Technology |
5536 |
WB (1:1000) |
| Antibody |
Anti-Akt (rabbit polyclonal) |
Cell Signaling Technology |
9272 |
WB (1:1000) |
| Antibody |
Anti-phospho-Akt (Ser473) (rabbit monoclonal) |
Cell Signaling Technology |
4060 |
WB (1:1000) |
| Antibody |
Anti-ubiquitin (rabbit polyclonal) |
Dako |
Z0458 |
WB (1:1000) |
| Antibody |
Anti-actin (rabbit polyclonal) |
Sigma-Aldrich |
A2066 |
WB (1:10,000) |
| Antibody |
Anti-NF-κB p65 (rabbit monoclonal) |
Cell Signaling Technology |
8242 |
WB (1:1000) |
| Antibody |
Anti-NF-κB1 p105/p50 (rabbit monoclonal) |
Cell Signaling Technology |
13586 |
WB (1:1000) |
| Antibody |
Anti-histone H3 (rabbit monoclonal) |
Cell Signaling Technology |
4499 |
WB (1:1000) |
| Antibody |
Anti-GAPDH (rabbit polyclonal) |
Abcam |
9485 |
WB (1:10,000) |
| Commercial assay or kit |
Nuclear Extract Kit |
Active Motif |
40010 |
|
| Commercial assay or kit |
M.O.M. Mouse-on-moue Kit |
Vector Laboratories |
BMK-2202 |
|
| Commercial assay or kit |
MultiScribe reverse transcription kit |
Applied Biosystems |
4368814 |
|
| Chemical compound, drug |
Antigen Retrieval Citra (pH-6.0) |
Biogenex |
HK086-5K |
|
| Software, algorithm |
ImageJ |
ImageJ |
|
|
| Software, algorithm |
GraphPad Prism |
GraphPad Prism |
|
|
| Software, algorithm |
Matlab |
MathWorks |
|
|
| Software, algorithm |
Image Studio |
LI-COR |
|
|
| Sequence-based reagent |
Fbxo32_F |
IDT |
qPCR primers |
AACCGGGAGGCCAGCTAAAGAACA
|
| Sequence-based reagent |
Fbxo32_R |
IDT |
qPCR primers |
TGGGCCTACAGAACAGACAGTGC
|
| Sequence-based reagent |
Trim63_F |
IDT |
qPCR primers |
GAGAACCTGGAGAAGCAGCT
|
| Sequence-based reagent |
Trim63_R |
IDT |
qPCR primers |
CCGCGGTTGGTCCAGTAG
|
| Sequence-based reagent |
Mstn_F |
IDT |
qPCR primers |
CAGACCCGTCAAGACTCCTACA
|
| Sequence-based reagent |
Mstn_R |
IDT |
qPCR primers |
CAGTGCCTGGGCTCATGTCAAG
|
| Sequence-based reagent |
Foxo3_F |
IDT |
qPCR primers |
ATCGCCTCCTGGCGGGCTTA
|
| Sequence-based reagent |
Foxo3_R |
IDT |
qPCR primers |
ACGGCGGTGCTAGCCTGAGA |
| Sequence-based reagent |
Lc3b_F |
IDT |
qPCR primers |
cactgctctgtcttgtgtaggttg
|
| Sequence-based reagent |
Lc3b_R |
IDT |
qPCR primers |
tcgttgtgcctttattagtgcatc
|
| Sequence-based reagent |
Gabarapl1_F |
IDT |
qPCR primers |
catcgtggagaaggctccta
|
| Sequence-based reagent |
Gabarapl1_R |
IDT |
qPCR primers |
atacagctggcccatggtag
|
| Sequence-based reagent |
Bnip3_F |
IDT |
qPCR primers |
AGGGCTCCTGGGTAGAACTG
|
| Sequence-based reagent |
Bnip3_R |
IDT |
qPCR primers |
GCTGGGCATCCAACAGTATT
|
| Sequence-based reagent |
Becn1_F |
IDT |
qPCR primers |
AGCCTCTGAAACTGGACACG
|
| Sequence-based reagent |
Becn1_R |
IDT |
qPCR primers |
CCTCTTCCTCCTGGGTCTCT
|
| Sequence-based reagent |
Atg5_F |
IDT |
qPCR primers |
ggagagaagaggagccaggt
|
| Sequence-based reagent |
Atg5_R |
IDT |
qPCR primers |
gctgggggacaatgctaata
|
| Sequence-based reagent |
Gapdh_F |
IDT |
qPCR primers |
TGTGATGGGTGTGAACCACGAGAA
|
| Sequence-based reagent |
Gapdh_R |
IDT |
qPCR primers |
GAGCCCTTCCACAATGCCAAAGT
|
