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. 2023 Jan 24;12:e84400. doi: 10.7554/eLife.84400

Figure 3. Remodeling of Sulfitobacter D7 transport systems in response to dimethylsulfoniopropionate (DMSP) and Emiliania huxleyi-derived metabolites.

Heatmap of gene expression of representative transport genes for various metabolite classes. Each row represents one gene, and the blocks represent a complete transport system in which at least two genes were differentially expressed (DE) in the comparisons indicated on the bottom. The column ‘cluster number’ corresponds to the heatmap cluster in Figure 1d in which the gene is found. Colored * denotes in which comparison of the gene was significantly DE. Color intensity corresponds to the standardized expression across all samples (triplicates of each treatment are shown). Expression values are scaled by row. Expression and fold-change values are presented in Figure 3—source data 1. ABC, ATP-binding cassette; TRAP, tripartite ATP-independent periplasmic; BCCT, betaine/carnitine/choline transporter. The letters correspond to the transport system components: A, ATP-binding; S, substrate-binding; P, permease; Q, dctQ subunit; M, dctM subunit.

Figure 3—source data 1. Sulfitobacter D7 expression of DE transport genes.

Figure 3.

Figure 3—figure supplement 1. Differential expression of Sulfitobacter D7 transport genes in coexistence and pathogenic states and in response to dimethylsulfoniopropionate (DMSP).

Figure 3—figure supplement 1.

Venn diagrams showing the number of differentially expressed transport genes in the indicated treatment comparisons. The left diagram corresponds to genes that were upregulated in samples with higher DMSP concentration, and the right side is downregulated genes. Venn diagrams were generated using BioVenn (Hulsen et al., 2008). CM, conditioned media; MM, minimal media; Exp-CM, exponential CM; Stat-CM, stationary CM.