Fig. 3.

Gut microbiota compositions at the Phylum and Family levels. (A–L) The microbiota compostion of stool conetnts from group 1 for “AOM/DSS and TP addition” criteria (A–C, G–I) and group 2 for “sex and ORX” criteria (D–F, J–L) at the Phylum (A–F) and Family (G–L) levels. Mann-Whitney U test for comparison difference between independent two groups was performed. ^†p < 0.05, female vs. male, ^‡p < 0.05, male vs. ORX, ^§p < 0.05, female vs. ORX in CON, AOM/DSS, and AOM/DSS+TP mice within group 2. ^a)p < 0.05, CON vs. AOM/DSS, ^b)p < 0.05, AOM/DSS vs. AOM/DSS+TP in female, male, and ORX mice within group 1. (M–O) Firmicutes/Bacteroidetes ratio (M) calculated by dividing the abundance of Firmicutes (N) with that of Bacteroidetes (O) in female, male, and ORX mice. Data are expressed as the mean±SEM. Whiskers show the minimum and maximum values. The p-values were calculated from the Mann-Whitney U test for comparison difference between independent two groups. *p < 0.05 for “CON vs. AOM/DSS” or “AOM/DSS vs. AOM/DSS+TP” in female, male, and ORX groups, ^†p < 0.05 for “Female vs. Male”, ^‡p < 0.05 for “Male vs. ORX”, ^§p < 0.05 for “Female vs. ORX” in CON, AOM/DSS, and AOM/DSS+TP subgroups. AOM, azoxymethane; CON, control; DSS, dextran sulfate sodium salt; ORX, orchiectomized; SEM, standard error of the mean; TP, testosterone propionate.