Table 13.
Effect of interferences on the H-point standard addition method.
| λ | Regression equation | R 2 | Type of interferences | Amount of interferences (µg/mL) | HCT (µg/mL) | CAN (µg/mL) | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Add | Found | %E | Add | Found | %E | ||||||
| 283 | Y = 0.01202∗X + 0.5936 | 0.9946 | Polyethylene glycol | 100 | 15 | 14.98 | 0.15 | 15 | 15.08 | 0.51 | |
| 239 | Y = 0.03424∗X + 0.9286 | 0.9901 | |||||||||
|
| |||||||||||
| 283 | Y = 0.01219∗X + 0.6102 | 0.9923 | Sucrose | 100 | 15 | 15.6 | 4.07 | 15 | 14.8 | −1.35 | |
| 239 | Y = 0.03424∗X + 0.9365 | 0.9901 | |||||||||
|
| |||||||||||
| 283 | Y = 0.01155∗X + 0.6039 | 0.9902 | Lactose | 100 | 15 | 15.5 | 3.4 | 15 | 15.3 | 2 | |
| 239 | Y = 0.03353∗X + 0.9402 | 0.9938 | |||||||||
|
| |||||||||||
| 283 | Y = 0.01165∗X + 0.5931 | 0.9965 | Starch | 100 | 15 | 14.96 | 0.29 | 15 | 15.6 | 3.74 | |
| 239 | Y = 0.03449∗X + 0.9485 | 0.9995 | |||||||||
|
| |||||||||||
| 283 | Y = 0.01115∗X + 0.5776 | 0.9974 | NaHCO3 | 100 | 15 | 14.95 | 0.34 | 15 | 14.89 | −0.74 | |
| 239 | Y = 0.03320∗X + 0.9059 | 0.9993 | |||||||||
|
| |||||||||||
| 283 | Y = 0.01197∗X + 0.6095 | 0.9967 | All of above | 100 | 15 | 15.5 | 3.4 | 15 | 15.23 | 1.54 | |
| 239 | Y = 0.03407∗X + 0.9461 | 0.9996 | |||||||||
∗ 1 calculated using the regression equation of Y = 0.0247x + 0.0297 and the HCT calibration curve at 283 nm.