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. Author manuscript; available in PMC: 2023 Jan 24.
Published in final edited form as: Sci Transl Med. 2022 Aug 17;14(658):eabj2681. doi: 10.1126/scitranslmed.abj2681

Fig. 2. Sphk2 deletion in renal perivascular cells ameliorates kidney fibrosis in the folic acid mouse model.

Fig. 2.

(A) Protocol for inducing kidney fibrosis by injecting folic acid (for B to E). Sphk2PVCWT and Sphk2PVCKO mice were given folic acid (250 mg/kg, ip) and euthanized at day 14. (B) Representative Masson’s trichrome staining of collagen in kidney sections at day 14. (C) Picrosirius red staining of kidney (polarized microscopy) with quantification of red/yellow birefringence of mature collagen fibers as a percentage of the total surface area of kidney section at day 14. n = 4 to 13 per group. (D) Acta2, Col1a1, and Col3a1 transcript expression (from whole kidney) at day 14. n = 4 to 13 per group. (E) Blood urea nitrogen (BUN) concentrations at day 14. n = 4 to 13 per group. Scale bars, 1 mm (B) and 200 μm (C). Data are represented as means ± SEM. *P < 0.05 and **P < 0.01; two-way ANOVA followed by post hoc multiple comparisons test (Tukey’s).