TABLE 1.
q‐PCR primers
| Target gene | Forward primers, 5′‐3′ | Reverse primers, 5′‐3′ |
|---|---|---|
|
miR‐942‐5p miR‐455‐5p LINC01018 |
GTACTCACAGCCCCTCACAC TGCCTTTGGACTACATCGGT GTAAATGAGGCTCCTGGGA |
CGGCAATTGCACTGGATACG TGTCGTGGAGTCGGCAATTG ATATTCTGTCTCCACCGCC |
| KNG1 | TGCTCCAGGCTGCTACTAAGT | GGCTTCAGTTATGCGGTACAA |
| SNAIL1 | TCGGAAGCCTAACTACAGCGA | AGATGAGCATTGGCAGCGAG |
|
TWIST1 Vimentin ZEB1 N‐Cadherin E‐Cadherin ZO1 U6 GAPDH |
GTCCGCAGTCTTACGAGGAG GACGCCATCAACACCGAGTT GATGATGAATGCGAGTCAGATGC TCAGGCGTCTGTAGAGGCTT CGAGAGCTACACGTTCACGG CAACATACAGTGACGCTTCACA CTCGCTTCGGCAGCACA GGAGCGAGATCCCTCCAAAAT |
GCTTGAGGGTCTGAATCTTGCT CTTTGTCGTTGGTTAGCTGGT ACAGCAGTGTCTTGTTGTTGT ATGCACATCCTTCGATAAGACTG GGGTGTCGAGGGAAAAATAGG CACTATTGACGTTTCCCCACTC AACGCTTCACGAATTTGCGT GGCTGTTGTCATACTTCTCATGG |