Extended Data Fig. 3. Cell-type fraction estimations and interaction model.

a) Validation heatmap of estimated cell-type fractions with clinical complete blood counts. The x axis shows the literature reference dataset used for the deconvolution procedure and the y axis is the cell-type fractions validated. The colors represent the Pearson correlation (rho) values between the estimated cell-type fractions and the corresponding complete blood count from the clinical data. The correlation values and associated 2-sided P values adjusted for multiple testing (FWER, Holm’s method) and are noted in each box. Some reference data sets did not include neutrophils (indicated by gray boxes). b) Estimated cell-type fraction variance explained by biological and technical variables. The x axis is the percent of variance of the cell-type fractions explained by covariates (colors) and the y axis the cell type assessed. Cell types are ordered by the decreasing percent of their variance explained by COVID-19 severity. The black dashed line represents the cutoff for inclusion in the cell-type-specific analyses. c) Schematic of interaction model for mock genes A and B. The x axis is the cell-type fraction of a specific cell-type of interest and the y axis the gene expression in log₂(counts per million). The color represents the presence (red) and absence (blue) of a symptom. The left and right facets show a gene not differentially expressed (same slope) and a differentially expressed gene (different slopes) respectively.