Table 1.
ANOSIM results for codon usage distance comparisons among the samples from seven selected habitats.
| Habitat1 | N1 | Habitat2 | N2 | R values for absCUFs based on Bray-Curtis dissimilarity | R values for absCUFs based on Euclidean distance | R values for synCUFs based on Bray-Curtis dissimilarity | R values for synCUFs based on Euclidean distance |
|---|---|---|---|---|---|---|---|
| Digestive system | 122 | Oral | 27 |
R = 0.1858; P = 2.00E-03 |
R = 0.1912; P = 1.10E-03 |
R = 0.1561; P = 4.60E-03 |
R = 0.1883; P = 1.10E-03 |
| Digestive system | 122 | Sediment | 60 |
R = 0.3365; P = 1.00E-04 |
R = 0.3469; P = 1.00E-04 |
R = 0.2242; P = 1.00E-04 |
R = 0.2231; P = 1.00E-04 |
| Digestive system | 122 | Skin | 15 |
R = 0.5845; P = 1.00E-04 |
R = 0.6208; P = 1.00E-04 |
R = 0.4337; P = 1.00E-04 |
R = 0.4556; P = 1.00E-04 |
| Digestive system | 122 | Soil | 85 |
R = 0.7576; P = 1.00E-04 |
R = 0.7774; P = 1.00E-04 |
R = 0.6751; P = 1.00E-04 |
R = 0.6608; P = 1.00E-04 |
| Digestive system | 122 | Wastewater | 45 |
R = 0.2951; P = 1.00E-04 |
R = 0.3162; P = 1.00E-04 |
R = 0.1898; P = 2.00E-04 |
R = 0.2087; P = 1.00E-04 |
| Digestive system | 122 | Water | 68 |
R = 0.4165; P = 1.00E-04 |
R = 0.4193; P = 1.00E-04 |
R = 0.3597; P = 1.00E-04 |
R = 0.3842; P = 1.00E-04 |
| Oral | 27 | Sediment | 60 |
R = 0.3362; P = 1.00E-04 |
R = 0.3368; P = 1.00E-04 |
R = 0.3281; P = 1.00E-04 |
R = 0.3368; P = 1.00E-04 |
| Oral | 27 | Skin | 15 |
R = 0.3827; P = 1.00E-04 |
R = 0.4215; P = 1.00E-04 |
R = 0.2901; P = 2.00E-04 |
R = 0.3237; P = 1.00E-04 |
| Oral | 27 | Soil | 85 |
R = 0.7415; P = 1.00E-04 |
R = 0.7399; P = 1.00E-04 |
R = 0.7046; P = 1.00E-04 |
R = 0.6935; P = 1.00E-04 |
| Oral | 27 | Wastewater | 45 |
R = 0.2428; P = 1.00E-04 |
R = 0.2553; P = 1.00E-04 |
R = 0.2447; P = 1.00E-04 |
R = 0.253; P = 1.00E-04 |
| Oral | 27 | Water | 68 |
R = 0.0415; P = 1.38E-01 |
R = 0.0272; P = 2.21E-01 |
R = 0.0311; P = 1.85E-01 |
R = 0.0396; P = 1.50E-01 |
| sediment | 60 | skin | 15 |
R = 0.3615; P = 1.00E-04 |
R = 0.3929; P = 3.00E-04 |
R = 0.2888; P = 8.00E-04 |
R = 0.3367; P = 1.00E-04 |
| sediment | 60 | Soil | 85 |
R = 0.3135; P = 1.00E-04 |
R = 0.3134; P = 1.00E-04 |
R = 0.3013; P = 1.00E-04 |
R = 0.2941; P = 1.00E-04 |
| sediment | 60 | Wastewater | 45 |
R = 0.0738; P = 3.60E-03 |
R = 0.0827; P = 1.00E-03 |
R = 0.0624; P = 7.60E-03 |
R = 0.0717; P = 3.40E-03 |
| sediment | 60 | Water | 68 |
R = 0.3768; P = 1.00E-04 |
R = 0.3667; P = 1.00E-04 |
R = 0.3807; P = 1.00E-04 |
R = 0.3862; P = 1.00E-04 |
| skin | 15 | Soil | 85 |
R = 0.502; P = 1.00E-04 |
R = 0.5085; P = 1.00E-04 |
R = 0.4354; P = 1.00E-04 |
R = 0.4419; P = 1.00E-04 |
| skin | 15 | Wastewater | 45 |
R = 0.3093; P = 3.00E-04 |
R = 0.3341; P = 4.00E-04 |
R = 0.2612; P = 5.00E-04 |
R = 0.3117; P = 2.00E-04 |
| skin | 15 | Water | 68 |
R = 0.3738; P = 1.00E-04 |
R = 0.3997; P = 1.00E-04 |
R = 0.3266; P = 1.00E-04 |
R = 0.3516; P = 1.00E-04 |
| Soil | 85 | Wastewater | 45 |
R = 0.4369; P = 1.00E-04 |
R = 0.4381; P = 1.00E-04 |
R = 0.4162; P = 1.00E-04 |
R = 0.4127; P = 1.00E-04 |
| Soil | 85 | water | 68 |
R = 0.7046; P = 1.00E-04 |
R = 0.6968; P = 1.00E-04 |
R = 0.7013; P = 1.00E-04 |
R = 0.6997; P = 1.00E-04 |
| wastewater | 45 | Water | 68 |
R = 0.2847; P = 1.00E-04 |
R = 0.2854; P = 1.00E-04 |
R = 0.291; P = 1.00E-04 |
R = 0.2971; P = 1.00E-04 |
For each sample, codon usage frequencies were calculated by two methods absCUFs and synCUFs (see main text). ANOSIM test was carried out considering the codon usage frequencies (absCUFs and synCUFs) of samples from two habitats at a time using Euclidean distance and Bray-Curtis dissimilarity matrices separately with 10,000 permutation values. Therefore, there are two columns for the R-values (one for Euclidean distance and another for Bray-Curtis dissimilarity). Here N1 and N2 represent the number of samples in the first and second habitat, respectively. ANOSIM test statistics are shown with R values and significance levels are shown with P-values.