Fig. 8. Exogenous addition of polyamines causes EIF5A upregulation, EIF5A hypusination leading to induction of T_regDys cells and their proliferation.

HTOC CD4⁺ T cells were TCR stimulated in the presence of GC7 (10 μM), Putrescine dihydrochloride (100 μM), or Spermidine (1 mM) without infection. Flow cytometry histogram overlays (top) and statistical quantification of MFIs (bottom) based on the flow cytometry analysis of ODC-1 (A), EIF5A (B), Hypusinated-EIF5A (C), AREG (D), and KI-67 (E) staining 6 days after stimulation. Means from triplicate experiments are plotted. ****P < 0.0001; ***P < 0.0006; **P = 0.002; *P < 0.01; Two-tailed; Unpaired t test in A–E. Circles within box plots represent each replicate.