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. 2023 Jan 24;14:381. doi: 10.1038/s41467-023-35802-y

Fig. 5. EXO1 responds to formaldehyde-induced DSBs.

Fig. 5

a IF staining against γH2AX (pSer 139) in S-phase indicated by EdU staining in RPE-1 WT and EXO1 KO (KO 7, KO 11) cells without treatment or treated with 100 µM formaldehyde for 18 h or 5 Gy irradiation. b Quantification of γH2AX (pSer 139) foci in S-phase cells for a. Data are shown with mean ± SEM from three independent experiments. ns: non-significant, *p < 0.1, ****p < 0.0001 (one-way ANOVA, followed by Kruskal–Wallis test). c IF staining against γH2AX (pSer 139) in S/G2-phase indicated by Geminin staining in RPE-1 WT and EXO1 KO 11 cells transfected with siCTL or siMus81, treated with or without 100 µM formaldehyde for 18 h. d Left. Protein levels of EXO1, Mus81 in RPE-1 WT and EXO1 KO 11 cells transfected with siCTL or siMus81,with α-tubulin as a loading control. Right. Quantification of γH2AX (pSer 139) foci in S/G2-phase cells for c. Data are shown with mean ± SEM from three independent experiments. ns: non-significant, ****p < 0.0001 (one-way ANOVA, followed by Kruskal–Wallis test).