Fig. 3.
Calculation of biological age (BA) based on age-dependent biomarkers. Fifteen biomarkers were selected based on prior knowledge of their age-dependence and availability in ARIC study. a, b) Verification of age-dependence for 15 biomarkers measured in ARIC study participants at visit 2 (n = 6,956, see methods and Fig. S1 for exclusions criteria). a) Linear regression lines for nine biomarkers that showed significant correlation with age and were used for BA calculations. 95% CI for the regression lines are shown in blue. rage denotes Pearson Coefficient for correlation of each biomarker with age (∗∗∗P < 0.0001). Pslope denotes P-value for the slope deviation from zero. The panel inserts show distribution of the study participants based on the corresponding biomarkers. b) rage and Pslope for six additional biomarkers with lower correlation coefficients that were included for BA calculation based on 15 biomarkers. c) Distributions of the study participants based on their chronological age (Age) and BA calculated from 9 to 15 biomarkers using Klemera and Doubal's method (see methods section for details). Distributions of BA from nine and 15 biomarkers are almost identical with Pearson correlation r = 0.99 (†P < 0.0001) indicating that they gave similar results.