Fig. 9. LDLR or SREBP-1‑deficient attenuates renal lipid deposition induced by long-term statins administration in STZ-induced diabetic mice.

All mice were ~30 weeks old. a Lipid profile and serum creatinine in STZ-induced diabetic mice. n = 3 in each group. Data are expressed as means ± SEM. b Representative images of Nile red staining. The lipid drops were stained in red, and the redder dots in the figure, the more lipid accumulation. Arrows represent lipid drops in renal tubular epithelial cells. Original magnification ×400. Scale bar: 50 µm. c Representative images of PAS staining. Original magnification ×1000. Scale bar: 20 µm. Arrows represent mesangial expansion. d Representative images of Masson’s trichrome staining. In the picture, collagenous components are stained as blue color and cytoplasm is varying shades of red. Collagen deposits (blue) are evident within the fibrotic interstitial lesions between tubular, and even in the glomerulus, as marked by arrows. Original magnification ×400. Scale bar: 50 µm. e–g Quantification of b–d staining. All image part of the kidney was cortex. Data are expressed as means ± SEM. n = 3 in each group. One-way ANOVA with Tukey post hoc test was used for the analysis of statistical significance. Source data are provided as a Source Data file.