Table 1.

PCR conditions and oligonucleotide sequences of F18 adhesive fimbriae and VTe2 (B-subunit) encoding genes

Gene	Accession number (GenBank)	Size (pb)	Primer sequence (5’ to 3’)	PCR conditions
F18 adhesive fimbriae	AJ308332.1	519	5’GATCCATGAAAAGACTAGTGTTTATTTCTTTTG 3’CGAATGCGCCAATGAATGTTCATTCTCGAG	Den. 95 °C 1’ ann. 56 °C 1′20’’ ext. 72 °C 1′30’’ 35 cycles
VTe2 (B-subunit)	GU459254.1	270	5’GGATCCATGAAGAAGATGTTTATAGCGG 3’AACGGGTCCACTTCAAATGATTCTCGAG	Den. 95 °C 1’ ann. 50 °C 1′20’’ ext. 72 °C 1′30’’ 35 cycles