Table 2.
Primer sequences and relative amplicon dimensions
| Gene1 | Accession number (GenBank) | Size (pb) | Primer sequence (5’ to 3’) |
|---|---|---|---|
| βactin F | DQ845171 | 76 bp | CTACGTCGCCCTGGACTTC |
| βactin R | DQ845172 | GCAGCTCGTAGCTCTTCTCC | |
| IL-6 F | JQ839263 | 112 bp | TGGGTTCAATCAGGAGACCT |
| IL-6 R | JQ839264 | CAGCCTCGACATTTCCCTTA | |
| IL-10 F | L2001 | 105 bp | TGAAGAGTGCCTTTAGCAAGCTC |
| IL-10 R | L2002 | CTCATCTTCATCGTCATGTAGGC | |
| COX1 F | EF568726 | 102 bp | GGAGCGGGTACTGGATGAAC |
| COX1 R | EF568726 | CACCTGCAAGGGTGTAGGGAGL | |
| COX2 F | AF304201 | 141 bp | AAGACGCCACTTCACCCATC |
| COX2 R | AF304201 | TCCATTGTGCTAGTGTGTGTCA | |
| GPx2 F | DQ898282 | 103 bp | GGAGATCCTGAACAGCCTCA |
| GPx2 R | DQ898282 | GCGAAGACAGGATGCTCATT | |
| LOX F | NM_001164001 | 112 bp | GTGGAGCACGAAAGCAAGACCC |
| LOX R | NM_001164001 | AAGGTGGGGTATGCATCGACAC | |
| NQ01 F | NM_001159613 | 118 bp | ATCACAGGTAAACTGAAGGACCC |
| NQ01 R | NM_001159613 | GCGGCTTCCACCTTCTTTTG | |
| CLAUDIN1 F | NM_001244539 | 90 bp | TCTTTCTTATTTCAGGTCTGGCT |
| CLAUDIN1 R | NM_001244539 | ACTGGGGTCATGGGGTCATA | |
| OCCLUDIN F | NM_001163647 | 106 bp | GTCCACCTCCTTATAGGCCTGATG |
| OCCLUDIN R | NM_001163647 | CGCTGGCTGAGAAAGCATTGG |
1CTB actin beta, IL-6 interleukin-6, IL-10 interleukin 10, COX1 cytochrome c oxidase subunit I, COX2 cytochrome c oxidase subunit II, LOX lysyl oxidase, GPX2 glutathione peroxidase 2, NQ01 NAD (P) H quinone dehydrogenase 1, CLDND1 claudin domain containing 1, OCLN occludin