Table 1.
Details of possible E chromosome sequences segregating among families
| Locus name | PCR product | Primer | Tm | GenBank accession hits | GenBank annotation |
|---|---|---|---|---|---|
| HFE174 | 330 bp | L: GTGGAAGAACAGTTGCAGCG | 60.041 | XP_031619250.1 | Uncharacterized protein LOC116338260 [Contarinia nasturtii]; jerky protein homolog-like [Epinephelus lanceolatus] |
| R: GCCGGAGATGGTTTGACCTT | 60.323 | XP_033472895.1 | |||
| HFE315 | 284 bp | L: GGAGGCATTCTCTCCGCAAT | 60.179 | RYE18558.1 | Reverse transcriptase family protein, partial [Sphingobacteriaceae bacterium]; RNA-directed DNA polymerase from mobile element jockey [Araneus ventricosus] |
| R: TGCGCTTGTTTCCTAGTTCCT | 59.928 | GBM72862.1 |