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. 2023 Jan 24;13:1326. doi: 10.1038/s41598-023-28062-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Schematic illustration of the sgRNA sequence cloned into the pX459 vector used to generate GPER knockout (KO) in MDA-MB-231 cells. (b) Protein expression levels of GPER in GPER (WT) and GPER (KO) MDA-MB-231 cells, as evaluated by western blotting analysis. β-Actin served as loading control. Immunoblots shown are representative of three independent experiments. Original blots are presented in Supplementary Fig. 1. GPER (WT) and GPER (KO) (c) MDA-MB-231 cells are treated for 72 h with either vehicle or increasing concentrations of ERα17p and peptide 2. The growth of cells receiving vehicle is set as 100%, upon which the viability of cells treated with ERα17p and peptide 2 is calculated. Values shown are mean ± SD of three independent experiments performed in triplicate. (*) p < 0.05.