Figure 2.

DGKB overexpression suppresses radioresistance and prolongs overall survival in GBM xenograft mouse models

(A) The changes in cell viability after IR or IR combined with DGKB overexpression in U87MG-RR, DGKB knockdown or knockout in U87MG, and DGKB knockdown in BCL20-HP02 and BCL21-HP03. Data are represented as mean ± SEM of three biological replicates.

(B) The changes in colony formation after shDGKB alone or DGKB overexpression alone, IR alone, or IR combined with DGKB overexpression in U87MG-RR, DGKB knockdown or knockout in U87MG, and DGKB knockdown in BCL20-HP02 and BCL21-HP03. Data are represented as mean ± SEM of three biological replicates.

(C and D) In vivo bioluminescence images (C) and relative luminescence units (D) of orthotopic xenografts derived from U87MG-RR in the untreated (control) group, IR group, and IR combined with DGKB overexpressing group (n = 20).

(E and F) Representative H&E staining (E) and IHC staining for DGKB and cleaved caspase 3 (F) of orthotopic xenograft GBM mouse models. Data are represented as mean ± SEM of three biological replicates. Scale bars, 2,000 μm (E) or 50 μm (F).

(G) Survival plots of mice with orthotopic xenograft GBM with DGKB overexpression and without (control) or with indicated treatment (IR treatments started 7 days after xenograft). Statistical analysis was performed with Student’s t test for (A) one-way ANOVA plus a Tukey’s multiple comparisons test for (B and D) compared with luminescence values of IR alone at 28 days after irradiation for (D), and Log rank (Mantel-Cox) test for (G). NS, non-significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.