Figure 3.
Patients with immune-related arthritis have higher TH1/2 cells and lower percentages of naive CD4 T cells at baseline
(A) Representative KNetL plot of annotated CD4 T cell clusters.
(B) Mean percentage of cells in six CD4 T cell subsets across the different clinical outcomes: arthritis, pneumonitis, and thyroiditis at baseline (unpaired t test).
(C) Volcano plot highlighting the up-regulated and down-regulated genes in cluster 25 of patients with arthritis, with paired-dot plots comparing the RNA expression of upregulated genes between no-irAE and arthritis groups.
(D and E) Gene Ontology (GO) analysis for differential genes present in cluster 25 from patients with arthritis for enriched terms from Immune.MSigDB (D) and from the GWAS Catalog 2019 (E).
(F) Pathway analysis of differentially expressed genes in cluster 25 from patients with arthritis using integrative KEGG-String platform.
Statistical significance for unpaired comparisons was performed by Student’s t test applying Mann-Whitney test. Data are presented as mean ± SEM. p value, exact, two-tailed, the center lines denote the mean of SEM. ∗p < 0.05, ∗∗∗∗p < 0.0001.