REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC/Cyanine7 anti-human CD3 (clone HIT3A)	BioLegend	Cat# 300318; RRID: AB_314054
Alexa Fluor® 700 anti-human CD4 (clone RPA-T4)	BioLegend	Cat# 300526; RRID: AB_493743
PE/Cyanine7 anti-human CD8a (clone RPA-T8)	BioLegend	Cat# 301012; RRID: AB_314130
Brilliant Violet 605™ anti-human CD45RA (clone HI100)	BioLegend	Cat# 304134; RRID: AB_11126164
Alexa Fluor® 488 anti-human CD27 Recombinant antibody	BioLegend	Cat# 393204; RRID: AB_2750088
BD Horizon™ BUV496 Mouse Anti-Human CD8	BD Biosciences	Cat# 612942; RRID: AB_2870223
BD Horizon™ BUV805 Mouse Anti-Human CD3	BD Biosciences	Cat# 612895; RRID: AB_2870183
BD Horizon™ BV750 Mouse Anti-Human CD4	BD Biosciences	Cat# 566355; RRID: AB_2744426
BD OptiBuild™ BUV395 Mouse Anti-Human CD45RA	BD Biosciences	Cat# 740298; RRID: AB_2740037
BD Horizon™ BV786 Rat Anti-Human CCR7 (CD197)	BD Biosciences	Cat# 563710; RRID: AB_2738384
CITEseq antibodies
TotalSeq™-C0072 anti-human CD4 Antibody	BioLegend	Cat# 300567; RRID: AB_2800725
TotalSeq™-C0046 anti-human CD8 Antibody	BioLegend	Cat# 344753; RRID: AB_2800922
TotalSeq™-C0154 anti-human CD27 Antibody	BioLegend	Cat# 302853; RRID: AB_2800747
TotalSeq™-C0063 anti-human CD45RA Antibody	BioLegend	Cat# 304163; RRID: AB_2800747
Chemicals, peptides, and recombinant proteins
DNase I	STEMCELL Technologies	Cat# 7470
Zombie UV™ Fixable Viability Kit	BioLegend	Cat# 423107
Critical commercial assays
Chromium Single Cell 5′ Library & Gel Bead Kit	10x Genomics	Cat# 1000006
RNA Isolation Kit	Qiagen	Cat# 74104
Software and algorithms
FlowJo 10.1r7	https://www.flowjo.com	SCR_008520
Single cell RNAseq analysis	https://github.com/rezakj/iCellR	N/A
GraphPad Prism 9	https://www.graphpad.com	SCR_002798
InnateDB	https://www.innatedb.com	SCR_006714
ShinyGO v0.76	http://bioinformatics.sdstate.edu/go	SCR_019213
Enrichr	https://maayanlab.cloud/Enrichr	SCR_001575
Cell Ranger	https://support.10xgenomics.com/single-cell-vdj/software/pipelines/latest/what-is-cell-ranger	SCR_017344
Deposited data
RNA sequencing data	https://www.ncbi.nlm.nih.gov/geo	GSE159774