Figure 2.

In vitro co-culture of immune-evasive SC-islet cells with allogeneic human immune cells

(A and B) SC-islet cell survival when co-cultured with primary human PBMCs at 1:1 and 3:1 PBMC/SC-islet ratio. Data are presented as mean ± SD (n = 5 donors in technical triplicate). p values were determined by one-way ANOVA with Tukey’s post-hoc test, ∗p < 0.05.

(C) T cell ligand gene expression in IFN-γ-treated CD49a⁺ SC-β cells. Ligand expression is presented as fold change (log₂).

(D and E) Differential expression of T cell co-inhibitory and co-activating ligands in IFN-γ-treated CD49a⁺ SC-β cells. Ligand expression is presented as fold-change (log₂). p values were determined by Student’s t test.

(F) SC-β cell survival when co-cultured with primary human CD56⁺ NK cells at 1:1 and 10:1 NK:SC-β cell ratios. Data are presented as mean ± SD (n = 5 donors in technical triplicate). p values were determined by one-way ANOVA with Tukey’s post-hoc test, ∗p < 0.05.

(G) Schematic of in vivo NK cell cytotoxicity assay.

(H) Analysis of in vivo NK cell assay. NK and SC-islet cell mixtures (3:1) were transplanted subcutaneously in Scid/beige mice (n = 5/group), and all were monitored by bioluminescence imaging on days 1 and 5 post-transplantation. Data are presented as mean ± SEM. p values were determined by two-way ANOVA, ∗p < 0.05. Dashed line represents background luminescence.