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. Author manuscript; available in PMC: 2024 Apr 1.
Published in final edited form as: Anesth Analg. 2022 Jul 25;136(4):779–788. doi: 10.1213/ANE.0000000000006146

Figure 5. Ketamine decreases endosome membrane-bound Rab5 and Rab7 but increases cytoplasmic amounts of Rab5 and Rab7.

Figure 5.

A: Effects of ketamine on whole-cell Na-K ATPase and the internal loading control GAPDH. One band is shown for each experimental condition. B: Quantitation of western blot analysis of whole-cell Na-K ATPase, n = 5 biological variables. C: Western blot analysis of cytoplasmic and membrane proteins Rab5 and Rab7 and internal loading control proteins Na-K ATPase and GAPDH. One band is shown for each experimental condition. D–G: Quantitation of western blot analysis of cytoplasmic Rab5 (D), cytoplasmic Rab7 (E), membrane Rab5 (F), and membrane Rab7 (G), n = 5 biological variables in each group. One-way ANOVA and post-hoc analysis with Dunnett’s test were applied to analyze data in panels D–G. Error bars represent SD; * indicates P < 0.05 by statistical analysis; ns indicates no significant difference among groups (P > 0.05) by statistical analysis.