Fig. 6. DSS temporal signature projection onto a human intestinal IBD causal network.

Colonic DSS temporal disease signatures were projected onto a Bayesian gene-regulatory network built from genotype and gene expression data of colonic samples from patients with Crohn’s disease. a The 862 colitis-genes that mapped show statistically significant overlap with the 905 genes of a subnetwork previously associated to Crohn’s disease (263 common genes, −log(p) = 44). The visualized subnetwork shows these common genes, along with their first neighbors (undirected, 959 genes in total). The blue-white-red color scale reflects the disease vs. control logFC of the genes in the mouse model. Gray genes were not differentially expressed in colitis. b Gene-set enrichment analysis (GSEA) was performed on both the mouse signature and the human subnetwork genes for enrichment in hallmark signatures of the Molecular Signature Database (MSigDB). Among the unique 42 molecular signatures found to be enriched in any of the gene-lists, 32 are shared, 7 are mouse signature-specific and 3 human subnetwork-specific. The FDR values of enrichment for mouse and human can be seen in the scatterplot, where the size of the circles represents the average number of genes in the overlap and the blue color gradient reflects the average −log (FDR).