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. 2023 Jan 24;14:392. doi: 10.1038/s41467-023-36045-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–d Quantitative flow cytometry data of matured DCs (CD11c⁺CD86⁺) in inguinal lymph nodes (LNs, a), and percentage of infiltrated CD8⁺ T cells (b), ratios of CD8⁺/Treg cells (c), and percentage of cytotoxic T cells (CTLs, CD8⁺GZMB⁺, d) in primary (Prim) tumors of mice with cryosurgery and L (left side) tumors of mice with no cryosurgery (n = 3 mice). e Representative immunofluorescence images of infiltrated CTLs in primary (Prim) tumors from mice with cryosurgery (n = 3 mice). f Representative flow cytometry plots showing the percentage of effector memory T cell (T_EM, CD3⁺CD8⁺CD44⁺CD62L⁻) in blood (BL) collected from mice with cryosurgery and injection of one of the various formulations. g Quantitative flow cytometry data of T_EM in blood of mice with/without cryosurgery and injection of one of the different formulations (n = 3 mice). h CD8⁺/Treg ratios in the blood of mice with/without cryosurgery and injection of one of the different formulations (n = 3 mice). i Representative flow cytometry plots of Treg cells in blood collected from mice with cryosurgery and injection of one of the different formulations. j Quantitative flow cytometry data of CD8⁺/Treg ratios in distant (Dist) tumors of mice with cryosurgery and R (right side) tumors of mice without cryosurgery (n = 3 mice). All mice were injected with one of the indicated formulations. k Quantitative flow cytometry data of the percentage of infiltrated CTLs in Dist tumors of mice with cryosurgery and R (right side) tumors of mice without cryosurgery (n = 3 mice). All mice were injected with one of the indicated formulations. l Representative immunofluorescence images of infiltrated CTLs in Dist tumors from mice with cryosurgery on Prim tumors and injection of one of the different formulations (n = 3 mice). Statistical analyses were done using two-way ANOVA with Sidak’s post-test and correction for multiple comparisons. The experiments for e and I were repeated three times independently (n = 3 mice) with similar results. Data are presented as mean ± SD (a–d, g, h, j, k). Source data are provided as a Source Data file.