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. 2023 Jan 11;13:1080753. doi: 10.3389/fphar.2022.1080753

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Copyright © 2023 Huang, Xue, Chen, Huangfu, Wang, Xiong and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nissl staining of the brain after gavage treatment for 1 month. After gavage treatment for 1 month, total surviving neurons increased, and dark neurons decreased (DG, dentate gyrus; CA, cornu ammonis) (n = 3, each mouse provides three data, data are expressed as mean ± standard deviation). (A) Nissl staining of the cortex and hippocampus in WT, control, 2 mg/kg, 6 mg/kg group. (B) Quantitative histogram of cortical total surviving neuron statistics in mice. (C) Quantitative histogram of cortical dark neuron statistics in mice. (D) Quantitative histogram of hippocampal total surviving neuron statistics in mice. (E) Quantitative histogram of hippocampal dark neuron statistics in mice. (F) Histogram of surviving total neurons in different subdivisions of the mouse hippocampus. (G) Histogram of dark neurons in different subdivisions of the mouse hippocampus.