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. 2023 Jan 5;26(2):105939. doi: 10.1016/j.isci.2023.105939

Figure 2.

Figure 2

Loss of histone H3 lysine 23 acetylation in BRPF1−/− hESCs

(A) Morphology of WT and BRPF1−/− H1 hESCs cultured at different time points. Scale bar, 200 μm. qRT-PCR analysis of the expression level of BRPF1 in WT and BRPF1−/− H1 hESCs cultured at different time points. The significance level was determined by unpaired two-tailed Student’s t tests. ∗∗, p < 0.01. The data represent the mean ± SD (standard deviation) from three independent repeats (n = 3).

(B and C) Examination of the expression of the selected (B) pluripotent and (C) lineage genes in BRPF1−/− H1 hESCs cultured at different time points. The significance level was determined by unpaired two-tailed Student’s t tests. ∗∗, p < 0.01. The data represent the mean ± SD (standard deviation) from three independent repeats (n = 3).

(D) Examination of H3K23ac, H3K14ac, H3K4me3 or OCT4, NANOG in BRPF1−/− H1 hESCs cultured at different time points by western blot. All error bars throughout the figure represent the SD (standard deviation) from three independent replicates (n = 3).