Fig. 3:

Exosomal miR-21-5p from peripheral blood of vitiligo patients inhibits the melanogenesis of melanocytes. PIG1 cells were co-cultured with Patient-exo and transfected with miR-21-5p inhibitor for 24 h. (A) Melanin content in melanocytes was detected by a microplate reader. (B) Tyrosinase activity in melanocytes was detected by a microplate reader. (C) The mRNA levels of MITF, tyrosinase, TRP1 and TRP2 were assessed by RT-qPCR assay. (D) The protein levels of MITF, tyrosinase, TRP1 and TRP2 were assessed by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001